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These N39 triple-reporter organoids could be passaged at least six times in collagen (Supplementary Fig

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A previous report showed cardiac PLN downregulation and short-term improvements in cardiac contractility following hydrodynamic intravenous delivery of a locked nucleic acid (LNA)-modified gapmer ASO targeting PLN in mice with pressure overload induced HF 23

These negative effects of GPx1 overexpression, however, are associated with prolonged upregulation of GPx1 or its activity, as found in GPx1 transgenic models, or in models with sustained activation of Nrf2 to foster increased production and recycling of GSH

However, these existing separation methods have notable limitations

Dextromethorphan, an oral alternative with additional activity at sigma1 and nicotinic receptors, has shown antiallodynic and antihyperalgesic effects in preclinical models