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For TAM differentiation, monocytes were maintained in a 1:1 (v/v) mixture of CM from liver cancer cells and RPMI 1640 medium supplemented with 10% human serum (Sigma, #H5667), 1% P/S, and 20 ng/ml human macrophage colony-stimulating factor (M-CSF

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[DOI] [PubMed] [Google Scholar] 70.Krolow R., Arcego D.M., Noschang C., Weis S.N., Dalmaz C

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Powers, 1987)

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