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Treatment of methyl mercury poisoning in mice with 2,3-dimercaptosuccinic acid and other complexing thiols

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[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Yu G., Wang L.G., Yan G.R., He Q.Y

Expression of hsf-1::degron::gfp (JTL611) was not induced by heat shock, and neither auxin nor hsf-1 (RNAi) significantly lowered the intensity of the GFP signal in this genetic background

Then, careful layering of 2.6 mL each of 1.15 M, 0.86 M, and 0.25 M sucrose solutions was performed on the microsomal suspension, and this step gradient sucrose solution was centrifugated at 82,000 g for 3 h at 4 C