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Mitochondrial DNA mutations in etiopathogenesis of male infertility

Oxid Med Cell Longev 2016:4350965

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For fluorescent labelling of UB, Cys-UB and Cys-UB K0 (1 mM) were buffer-exchanged using a PD MidiTrap G-25 column (Cytiva, 28918008) into 25 mM HEPES, 150 mM NaCl and 0.5 mM TCEP and incubated with 5-(Iodoacetamido)fluorescein (5 mM, Sigma-Aldrich, I9271) for 18 h at 4 C