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The sites of deuterium-incorporated isoAsp in proteins can be unequivocally determined by comparing the precursor and product ion masses of the peptides from proteins reacted in H 2 O and D 2 O

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The fractions containing DAP BII were pooled, buffer-exchanged to 80 mM Tris/HCl pH 8.5 using a Vivaspin 20 concentrator (GE Healthcare) and concentrated to 10 mg/ml using the concentrator