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The final pellets were air-dried and resuspended in the appropriate sample buffers and then subjected to SDS-PAGE and two-dimensional (2D) gel electrophoresis, followed by Flamingo fluorescence gel staining analysis (Bio-Rad Laboratories, Hercules, CA) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) analysis at the University of Pittsburgh Health Sciences Mass Spectrometry Core

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