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Using these enzyme preparations with a defined GSH concentration (20 M) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig

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Similar content being viewed by others Log in or create a free account to read this content Gain free access to this article, as well as selected content from this journal and more on nature.com or Abbreviations EPO: erythropoietin HIF: hypoxia-inducible factor ODDD: oxygen-dependent degradation domain pVHL: von HippelLindau tumor suppressor protein References Semenza GL, Wang GL

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