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We examined this interaction among adult participants in phase 2 of the prefolic acid fortification NHANES III (19911994) and in the postfortification NHANES 19992002

For histological assessment, paraffin tissue sections were stained with hematoxylin and eosin (HE) and Safranin O-Fast Green (SOFG) according to standard laboratory protocols

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Merighi et al

The significant drop in testosterone may reflect direct damage to Leydig cells, while reduced FSH and LH levels suggest involvement of the hypothalamic-pituitary axis

[DOI] [PubMed] [Google Scholar] 51.Bouitbir J et al