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Description
Prior to in-solution enzymatic digestion with trypsin overnight at 25C (1:50 trypsin:protein ratio), sample solutions were evaporated to half of the starting volume by vacuum centrifugation to increase protein concentration maintaining SDC at a concentration tolerable for trypsin activity
10.1007/s00726-017-2436-z 23 ChoiB.-H.ColoffJ
Mol Psychiatry 2012
Science 312 , 12111214 (2006)
Every plants first line of defense amid stress is the ROS generation
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