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After washing with washing buffer (20 mM Tis- HCl, pH7.5, 150 mM NaCl, 10% Glycerol, 1% Nonidet P40 Substitute, 5 mM MgCl2, 2 mM EDTA, pH 8.0), the protein samples were denatured in 1 loading buffer

1A), the quenching efficiency of 5 mM sorbate is lower in the generation of O 2 ( 1 g ) that accompanies the HRP-catalyzed oxidation of IBAL

10.1016/j.cell.2025.03.011 190 KuniedaT.MinaminoT.MiuraK.KatsunoT.TatenoK.MiyauchiH.et al (2008)

Reconstitution and preparation Both BPC-157 and TB-500 come as lyophilized (freeze-dried) powders that must be reconstituted with bacteriostatic water before use