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The reviewer GM declared a shared affiliation, with no collaboration, with the authors to the handling editor at the time of the review

IGF-1 LR3 For In Vitro Research Use Only Not for Human or Veterinary Application For In Vitro Research Use Only Not for Human or Veterinary Application IGF-1 LR3 (Long Arg3 Insulin-like Growth Factor-1) is a synthetic 83-amino acid analog of native insulin-like growth factor-1 (IGF-1), incorporating a 13-amino acid N-terminal extension and a substitution of arginine for glutamic acid at position 3 of the mature IGF-1 sequence

doi: 10.1155/2015/783761

The protocol was as follows: (1) baseline (80 s ), (2) Valsalva maneuver (20 s), (3) normal breathing (160 s)