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However, without NADH and mitochondria present, MB increased DCF fluorescence at 100 nM, 1 M and 10 M (Figure 10A)

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For teams publishing AOD-9604 data, reporting the lot number, reconstitution diluent, and storage interval alongside assay results improves inter-laboratory comparability

In cells exposed to high levels of oxidative stress, like red blood cells (RBC), up to 10% of the glucose consumption may be directed to the pentose phosphate pathway (PPP) for the production of the NADPH needed for this reaction

Finally, as both increasing age and germ-free conditions have been associated with a decline Drosophila antioxidant responses 22,23 , we defined the requirement of the antioxidant response system in our model and find, in agreement with vertebrate studies, that genetic and pharmacologic manipulations of antioxidant response pathways results in altered sensitivity to APAP toxicity 24,25