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Immunol Res (2006) 35(12):12736.10.1385/IR:35:1:127 149 SaxtonTMHenkemeyerMGascaSShenRRossiDJShalabyFet alAbnormal mesoderm patterning in mouse embryos mutant for the SH2 tyrosine phosphatase Shp-2

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The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at 80C

Most of the evidence giving rise to this claim comes from a few observational studies

Local injection may provide faster onset at specific sites while systemic dosing offers broader distribution

This first step is also the most critical rate-limiting step