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After six days of culturing the cells together, they were collected and utilized in an assay to measure the viability of the MAP antigen ( 2.5.2 Preparation of macrophages for use as a target cell for MAP infection The monocytes isolated from PBMCs at 16 weeks post-inoculation were used for generating monocyte-derived macrophages

Further studies found that AUF1 interacts with the coding sequence and 3-untranslated region (UTR) of Nrf2 to stabilise the gene, whereas AUF1 binding to the 3-UTR of activating transcription factor 3 ( ATF3 ) promotes degradation of the gene, producing an anti-ferroptosis effect and ameliorating ALI [68]

Non-uniquely mapped reads were discarded

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Paeoniflorin also helps maintain cellular homeostasis by limiting pathological calcium influx and oxidative stress-induced damage (38)