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10.1038/ncomms8737 Nat Commun

Confluent cell cultures were washed once with PBS, and then incubated for 20 min at room temperature with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei, washed with PBS, permeabilized with 0.1% Triton-X 100 for 30 min, fixed at 37 C for 20 min with 4% Paraformaldehyde in PBS, and blocked with 2% BSA in PBS at room temperature for 20 min

step D, coronary flow velocity reserve (CFVR) in the left anterior descending artery

These have been studied for various health benefits, including improving gut health, boosting the immune system, and reducing the risk of certain diseases such as allergies, obesity, and diabetes

Central Nervous System Agents in Medicinal Chemistry, 23 (2), 137143