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For MBP-tag pulldowns, purified HisMBPAcP10, HisMBPAcP10 mutants, eIF2B() 2 , eIF2B, eIF2B( latch ) 2 , eIF2B latch and eIF2B 2 were incubated (with gentle rotation at 4 C) with amylose resin in Assay Buffer (20 mM HEPES-KOH pH 7.5, 150 mM KCl, 5 mM MgCl 2 , 1 mM TCEP, 1 mg ml 1 BSA and 5 mM CaCl 2 )

In effect, an oxidative intracellular environment may impair the function of Na + /H + antiporters, decreasing the intracellular pH and favoring an early phase of viral replication [84]

Kopec, F

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doi: 10.1038/s41419-020-2299-1 78 XuX.WuY.YiK.HuY.DingW.XingC