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Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein

An additional issue that may arise when the CBS gene is impaired is an excess accumulation of hydrogen sulfide gas (H2S) that may lead to gut dysbiosis or hydrogen sulfide SIBO

The transcript levels of genes encoding GPX-like ( GPX2 , GPX6 , GPX7 , GPX8 ) were analysed (Fig

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