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The supernatant was further centrifugated at 300,000 g for 50 min to get the membrane pellets, which were resuspended in buffer containing 20 mM HEPES pH7.5, 100 mM NaCl, 1 mM EDTA, 0.2 g ml 1 leupeptin, and 100 g ml 1 benzamidine and stored at 80 C
To et al
Rice Dr
The inhibition of Nrf2 accelerates renal lipid deposition through suppressing the ACSL1 expression in obesity-related nephropathy
