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Description
Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3/Snail signaling

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The practical takeaway is mechanistic: these enzymes are not standalone antioxidants, but mineral-dependent systems whose performance reflects availability, balance, and cellular demand (Jewell DE, 2024)

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Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)
