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Resolutions were estimated using the gold standard criterion (FSC = 0.143), and the local resolution was calculated in cryoSPARC

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When you are deficient in B12 your body becomes tired, and weak, which can lead to anemia

After washing once with PBS to remove unbound CFSE, SFN was added for 30 min and the cells were seeded on 96-well microplates coated with anti-CD3/CD28 antibodies for co-stimulation as described above

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Another research group (46) used a nebulizer to aerosolize saline or VG/PG with 0 or 18 mg/ml nicotine, exposing 12-wk-old A/J mice 5 days/wk for 4 mo