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EHMES-1 cells were stimulated by the respective dimeric macrocycles and the phosphorylation level was quantified by enzyme-linked immunosorbent assay (ELISA) using a rabbit pY1234/1235-specific mAb

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Common side effects during the infusion include: Chest tightness or pressure (caused by NAD+'s effects on smooth muscle) Facial flushing and warmth (vasodilation) Nausea (particularly at doses above 500mg) Anxiety or restlessness (likely related to sympathetic nervous system activation) These effects are transient, they resolve when the infusion ends or when the drip rate is slowed