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Micro slide 8-well glass bottom plates (Catalog 80807, Ibidi, Grfelfing, Germany) were treated with 50 L of a 1 mg/ml concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl 2 ,1 mM MnCl 2 , 0.01% NaN 3 , Catalog C2010, Sigma Aldrich) solution, incubated for 5 min and then washed twice with 50 L of fresh medium

316 Therefore, it is important to realize that while the classic arginine-dependent production of NO is dominant when oxygen tensions are high, when oxygen tension is low alternate NO 2 -dependent mechanisms are available and may contribute to nitro-FA production

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Upregulation of IGF-IR signaling can help cancer cells resist anoikis by inhibiting p53 and p21 activation [51]

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