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Specifically, the system consisted of a Thermo Q-Exactive in line with an electrospray source and an Ultimate3000 series HPLC consisting of a binary pump, degasser and auto-sampler outfitted with an Xbridge Amide column (Waters) (dimensions: 3.0 100 mm and a 3.5-m particle size)

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Proper peptide handling during preparation and storage helps reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation that may affect analytical performance in experimental systems

The primary difference lies in the absorption rate
Targeted construction of phosphorylation-independent beta-arrestin mutants with constitutive activity in cells
