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1999 Mar 1;136(2):195-201

[DOI] [PubMed] [Google Scholar] 73.Liepinsh E, Makrecka-Kuka M, Makarova E, Volska K, Svalbe B, Sevostjanovs E, et al
Quantitative real-time PCR (qRT-PCR) analysis was performed on a CFX 96 BioRad cycler in triplicate with SensiFAST SYBR Green Kit (Catalog Number BIO-98005, USA), according to manufacturers instructions

The problem I can see with this is, as well as being a bit of a faff, its difficult to measure the right amount
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Plan your dose increases for days when you have a lighter schedule
