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The OTU clusters and representative sequences were determined using the UPARSE algorithm 54 , followed by taxonomy assignment using the Silva database v128 55 and the RDP Classifier 56 with a bootstrap confidence cutoff of 80% performed by using QIIME v1.8.0 57

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For those suffering from vertebral fractures, identifying the cause is essential for determining the most effective treatment plan to relieve pain, promote healing, and prevent future fractures