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5.4 Rationale for hydrogen-assisted extraction (H2Ext) In recent years, H2Ext has emerged as a low-cost, non-toxic, and safe green extraction method (184)

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The phosphopeptides were eluted by adding 80 L of elution buffer directly (50% acetonitrile, 500 uL H 2 O, 7% Nh4OH ammonium hydroxide)

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This preparation of sterile water and benzyl alcohol (BnOH) allows repeated withdrawals to be made from a single 30mL plastic vial