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-Catenin siRNA loaded via lipofection mediates potent gene knockdown in vitro versus scrambled controls ( 6.3 Cargo loading Munagala et al first reported that MEVs, as drug delivery systems, load small drug molecule compounds, including curcumin, withaferin, anthocyanidins, paclitaxel (PAC), and docetaxel, targeting lung and breast cancer cells while enhancing anticancer and anti-inflammatory effects ( 6.4 MEV delivery methods Various strategies, including co-incubation, electroporation, and freeze-thaw cycles, have been used recently to load therapeutic molecules into MEVs

Nat Commun (2021) 12(1):6869