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Waltham, MA, USA) staining as adapted and modified from published protocols.[12] Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution (~0.2% oil red O in 99% isopropanol) for 30 min

Behavioral testing Force swim test (FST) A force swim test is widely employed for the evaluation of depression-like behaviors in rodents (Samad et al
Prog Neuropsychopharmacol Biol Psychiatry 30:969995 Young RC, Biggs JT, Ziegler VE, Meyer DA (1978) A rating scale for mania: reliability, validity and sensitivity

The damage caused by free radicals is capable of providing feedback to the system and triggering an oxidative storm, affecting the body as a whole