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Biochemical features Biochemical features in ferroptosis involve iron accumulation, lipid peroxidation, generation of ROS, and depletion of glutathione peroxidase 4 (GPX4)

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The following equation was used to calculate the spontaneous alternation percentage (SAP) based on the number of alternations and total arm entries: SAP (%) = [number of alternations/(total arm entries 2)] 100

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