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MuSC Proliferation In aged skeletal muscle, NNMT overexpression is linked to impaired NAD+ salvage, dysregulated SIRT1 activity, and increased muscle stem cell (MuSC) senescence
In brief, cells were washed once with ice-cold PBS before quenching with 300 l 2:1 ice-cold MeOH:H 2 O on dry ice
