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Secondary antibody was removed and membranes washed four times in PBS-T before addition of Luminata Forte (Millipore)

Pediatr Blood Cancer 2016, 63:13491356

ZL searched, analyzed and summarized the literature results

Preservatives Preservative- Picture courtesy: USADA This product is also suspectable to microbial growth and reproduction, so, preservatives, such as phenol, metacresol, and, benzyl alcohol are incorporated during its preparation to deter microbial presence

Each 20 mL PCR reaction contained 1 mL of 1/20-fold diluted cDNA, 1X Phusion GC buffer, 0.2 mM deoxynucleotide triphosphate (dNTP), 1 mM primers, 3% (v/v) DMSO, 0.5 M betaine, and 0.4 units Phusion High-Fidelity DNA polymerase (New England Biolabs)

That is a larger volume, which is why the TB-500 dosage calculator can help you plan reconstitution volumes that keep injection sizes manageable