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Glutathione is present in increased concentrations in alveolar fluid of patients with asthma, and an association exists between levels of glutathione and the degree of bronchial hyperresponsiveness

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Samples were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, #84868) into 8 fractions per sample and the resulting fractions were lyophilized at 37 C until completely dry

Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors