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NLC-LC (size 1443 nm, zeta 38.61.4 mV, encapsulation efficiency 562%) were incubated for 1 h at 0.5 mg/mL LC equivalent with density-gradient-purified spermatozoa from 25 asthenozoospermic men alongside free LC, blank NLC, and untreated controls

The morphology of the synthesised materials was confirmed and an electrode was prepared to perform a cyclic voltammetry (CV) in phosphate buffer saline (PBS - pH 7) medium to examine the redox behaviour SMX

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