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After protein quantification, the lysates were added with MDA working solution and heated at 95C for 15 minutes

Others prefer to mix it in a liquid, with orange juice being the most popular choice
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Nguyen CT, Gracely EJ, Lee BK

However, the contrasting nature of the results observed, particularly regarding the effects on cardiovascular morbidity and mortality in observational versus intervention studies, suggests that further research is warranted to support the repurposing of methotrexate for cardiovascular prevention (Table 1)
