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From the L4 stage, worms were transferred to NGMi plates with 5-Fluorouracil (5-FU, Sigma) and cultured at 25 C

This approach is commonly used to separate fragment behavior from broader endocrine effects in mechanistic studies

2024;223:430-442.[DOI] 163
The primary routes for permeation across the BBBinclude passive diffusion (lipid soluble solutes), ABC transporter efflux, carrier-mediated influx, and receptor-mediated transcytosis (Pandian et al., 2022

It plays a role in memory, focus, and mood stability while reducing the risk of nerve damage and cognitive issues
