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Eluted protein was further purified by HiTrap Heparin HP affinity column (Cytiva, 17040703) with a linear gradient of Low-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 50 mM NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) and High-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 1 M NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) to remove RNA contamination

Metwaly, A

Nuclear folate metabolism

Mitochondrial bax translocation accelerates DNA fragmentation and cell necrosis in a murine model of acetaminophen hepatotoxicity

TB-500 considerations: Animal studies found TB-500 could accelerate dormant tumor growth and affect immune responses

doi: 10.3390/toxins8070191