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Selected cells were maintained in DMEM plus medium (DMEM supplemented with 20% FCS, L-glutamine, 10 4 U/mL/10 4 g/mL penicillin/streptomycin, 0.8 mg/mL neomycin, and 1.0 g/mL puromycin)

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For instance, AARS1-mediated lactylation of p53 at lysines 120 and 139 suppresses the transcription of p21, and p53 upregulates modulator of apoptosis (PUMA) by disrupting p53 LLPS and its DNA-binding capability

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