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Briefly, at 19.5 h of treatment, 4 L of 2.5 mM CellROX Green was applied to each well for a working concentration of 5 M

It reaches the cells and bloodstream intact

Copper binding creates complex with antioxidant, anti-inflammatory, and tissue remodeling properties

Although the exact mechanisms mediating the onset of PD are unclear, PD development is mainly promoted by neurotoxic oligomers consisting of -synuclein, excessive ROS production, neuroinflammation and dopaminergic neuron loss

[DOI] [PubMed] [Google Scholar] 64

Cell lines were carefully selected for screening peptides as our goal here is to screen peptides with high binding/uptake by the TNBC cells
