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Specifically, extracts from the viscera of sea cucumbers (Stichopus japonicus viscera extracts, VF) have been shown to stimulate the ERK signaling pathway, downregulate melanin synthesis, and enhance collagen synthesis 42

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We then built the isobologram of the corresponding dose-response curves with a fixed dose ratio of R and compared the expected mixture of the two doses that produced an effect equal to half of the maximal effect produced by the individual drugs and to the experimental value (see later in methods)

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