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These antibodies were used for immunofluorescence staining: NeuN(1:500, Abcam, Cat# ab177487), IGF1(1:500, Abcam, Cat# ab9572), IBA1 (1:500, Abcam, Cat# ab5076), c-Fos(1:500, CST, Cat#2250), CGRP(1:1000, SCBT, Cat#sc-57053), p-IGF1r(1:100, Abclonal, Cat#AP0367)

Animal models of developmental thyroid hormone deficiency have also reproduced marked neurocognitive impairment and growth retardation in offspring, much like in humans with hypothyroidism (Chaulin et al

Intracellular lipids were stained with Oil Red O to analyse fat accumulation in the liver

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Research Disclaimer: Doses reported below are from published preclinical research protocols