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In the first series, concentration was slowly lowered to 5M M22, and the perfusate was then switched to 5M M22 fixative
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National incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 19902017: A systematic analysis for the global burden of disease study 2017
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The cells were cultured in DMEM/F-12 medium (11320033, Gibco, USA) supplemented with 5% horse serum and 2.5% fetal bovine serum at 37C under an atmosphere of 5% CO 2
