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The reaction can proceed (a) either in a mixed disulfide intermediate with an N-terminal thiol active site

The impact of glutathione on fever has been comprehensively reviewed in a recent review by Wrotek et al

Plotting the reciprocal of enzymatic rates by the reciprocal of substrate concentrations for each concentration of LCS3 produced lines of best fit that are parallela strong indication that LCS3 acts on both GSR and TXNRD1through an uncompetitive inhibitory mechanism

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Microbiol (Reading England) (2003) 149(Pt 3):66572

doi:10.1021/acs.orglett.6b02611