gst protein binding to glutathione Tagged and GST Tag Purification GST-His purification: A Two-step Affinity

Description

Amounts of harman and norharman levels are very low in green coffee and increase significantly during roasting, but are highly variable

Purity 99% Form Lyophilized Blue Powder Grade Research Grade Storage 28C / -20C

Dual Use: Functions as both an ice pack* and a drink bottle

Supports Detoxification

Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase (GSH1) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively