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After two rounds of selection, gene deletions were confirmed by PCR on genomic DNA using primers binding ~400 base pairs up- and down-stream of the gene of interest

To examine whether the therapeutic effect could be maintained after a suspension of treatment, administration of PEI or peptide was resumed and continued for 4 more months (experimental set 2, Appendix Fig S3)

In contrast, the study of GSH-Pxs in prokaryotes remains underexplored, and they have been reported only in Streptococcus pyogenes , where a gsh-px mutant was associated to an impaired virulence in mice infection models [13]

Shaking creates air bubbles and mechanical stress that can degrade the peptide