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Finally, although PLS-DA provided a robust visualization of group separation, we acknowledge that its application to a low-dimensional clinical dataset carries a risk of overfitting

This heightens susceptibility to the toxic effects of iron overload, disrupting intestinal mucosal homeostasis and triggering local inflammation and cell death, ultimately precipitating the onset of gut-associated diseases ( 2.2 Iron overload, deficiency, and IBD pathogenesis In the intestinal microenvironment of IBD, iron overload or functional iron deficiency not only serves as a source of oxidative stress but also acts as a critical factor triggering ferroptosis in intestinal epithelial cells

Furthermore, in synthetic polypeptides, the formation of the intramolecular -helical elements joined by flexible coils without the formation of a hydrophobic core does not affect the shape and intensity of the SAXS pattern including the shape of the Kratky plot (Semisotnov et al., 1996)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 10.Meimaridou E, Kowalczyk J, Guasti L, Hughes CR, Wagner F, Frommolt P, Nurnberg P, Mann NP, Banerjee R, Saka HN, Chapple JP, King PJ, Clark AJ, Metherell LA