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72 h after transfection, cells were harvested by trypsin digestion and re-suspended in 100 uL of 1 PBS

However, in tauopathies such as AD, as well as in hibernation, tau undergoes hyperphosphorylation catalyzed by various protein kinases, including but not limited to glycogen GSK3, cyclin-dependent kinase 5 (CDK5), and protein kinase A (PKA) [103,104,105], accumulating an excess of 7-10 phosphate groups [106]

doi: 10.1007/s00204-020-02718-1

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