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Next, the membranes were incubated with primary antibodies overnight at 4 C, followed by HRP-labeled mouse or rabbit secondary antibodies for 120 min at 4 C

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Sera were collected at baseline, 0.25 h, 0.5 h, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 6 h, 9 h, 12 h, 24 h, day 3, day 7, day 14, day 21, and day 30 for analysis of sUA and at baseline, day 14, day 21, and day 30 for analysis of ADAs