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The membranes were blocked with TS solution [150 mM NaCl, 20 mM Tris-HCl (pH 8.0)] containing 1% non-fat dry milk for 30 min at room temperature, and then incubated with a primary antibody overnight at 4C and probed with an HRP-conjugated secondary antibody (KPL, Inc.) for 1 h at room temperature

doi:10.1016/S0166-2236(00)02031-2 69

In animal and preliminary human studies, BPC 157 showed the ability to: Accelerate regeneration of muscles, tendons, and ligaments Support brain and nervous system function Influence the lining of the gut Reduce inflammation markers Support sleep quality Enhance mood and mental clarity Promote blood vessel formation in injured tissues Support bone density and connective tissue integrity All of this from something born in the stomach

KeshavarziAAkramiRZarshenasMMZareieSGhadimiTNajafiAet al

In more serious cases, it can also cause neuronal death [82, 83]

M., Weiner M