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The next day, sections were incubated for 1 h at room temperature with fluorescent secondary antibodies, including Donkey Anti-Goat IgG (1:1000, Jackson ImmunoResearch, 705-606-147, Alexa Fluor 647) and Goat Anti-Rabbit IgG H&L (1:1000, Abcam, ab150080, Alexa Fluor 594)

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Additional physiological issues such as dry eyes, dry mouth, and speech difficulties can further diminish overall health and quality of life