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RNA sequencing libraries were prepared with unique barcodes using the NEBNext Ultra RNA library kit for Illumina (New England Biolabs) according to manufacturers instructions

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doi: 10.1016/j.bjid.2016.09.002

Virus yield reduction assay The reduction in virus titer was assessed in medium after 2 days infection of Calu-3 in the Vero E6 cells by plaque assay

It is found that the concentration of taurine in the hippocampus may serve as a potential signal for the growth and beginning of major depressive disorder (MDD) (112)